From printed DNA to high-quality coding libraries

SynPlexity and Flock Bio combine printed DNA synthesis, plasmid library construction, and sequencing-based QC into a single streamlined workflow for building high-quality protein-coding libraries.

For a recent 786-member, 918 bp library at 70% sequence identity, the workflow achieved up to 99.2% intended design detection, with up to 98.1% of designs recovered as exact plasmid sequences. The resulting library also showed strong balance, with more than 80% of designs within 5x of the mean representation, and more than 50% perfect reads.

The result is a partner workflow designed to deliver what screening teams actually need: broad variant recovery, balanced library representation, and strong final-library sequence quality.

Reflecting on the collaboration, Craig Stolarczyk, CEO of SynPlexity, emphasized the broader vision behind the work. “The future of biology belongs to those who can explore gene pools at scale. SynPlexity’s NucleoPool platform generates pooled gene constructs with unprecedented diversity, and our collaboration with Flock Bio ensures researchers can propagate and unlock that potential.”

“I’m really excited about the new cost and diversity options that are enabled by our new partner, SynPlexity”, says Mat Falkowski, CEO of Flock Bio.  “This will greatly help MPRAs explore promoter and UTR sequence spaces beyond the typical 150-400nt oligo pools.”

Together, SynPlexity and Flock Bio provide a cost-effective, rapid-turnaround solution for building high-quality libraries from printed DNA to sequence-validated plasmids in as fast as <3 weeks. To learn more about the partnership and each company’s capabilities, visit synplexity.com and flockbio.com.